P-001 FORMING A LIVING TISSUE MODEL OF THE POSTERIOIR RECTUS SHEATH

Abstract Aim To develop an in-vitro model of the posterior rectus sheath. The should be formed living cells and aligned collagen that is a similar thickness tensile strength to Materials Methods Rectangular Fibroblast populated Colagen matrices (FPCMs) were using rat-tail seeded with immortal cell line human fibroblasts. FPCMs into 3D-printed tethered gel moulds where they at opposing ends induce formation construct. cultured for 10 days. After days, excess fluid was removed constructs plastic compressed under custom made compression blocks. Constructs fixed in 4% paraformaldehyde. underwent flouresence icroscopy, second harmonic generation microscopy uniaxial analysis. Results 6 developed. Lateral alignment both fibroblatss confirmed on respectively. Median construct after 0.57mm. Tensile analysis still awaited. Conclusion - here we present one first ever models posterioir sheath It represents novel potential development “living mesh” or shetah transplant. Our will further optimised coming months by introducing